Silley 2012 has argued that the terms resistant and sensitive should. Data from the canadian national gonococcal antimicrobial susceptibility comparison program, including results from 25 proficiency panels distributed between 2003 and 2012, were analyzed. National committee for clinical laboratory standards corporate author. Areas of technical uncertainties atu and information on how to handle unavoidable technical uncertainty in antimicrobial susceptibility testing.
The accuracy of the phoenix automated system in combination with 2012 eucast breakpoints against recent clinical isolates was evaluated. The clinical importance of antimicrobial susceptibility test results requires that these tests be. Buy performance standards for antimicrobial susceptibility testing. Performance standards for antimicrobial disk susceptibility tests. Educational susceptibility testing as a critical component. Determination of disk diffusion and mic quality control guidelines. Susceptibility testing of fastidious bacteria 21 6. The clinical and laboratory standards institute clsi subcommittee on antimicrobial. Quality assurance for antimicrobial susceptibility testing. A clinical and laboratory standards institute clsi m23style quality control qc. Laboratory standards institute antimicrobial susceptibility testing standards m02a12. Twentythird informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute.
See also the eucast aminoglycoside guidance document for the use of the revised aminoglycoside breakpoints. Susceptibility testing approved these mic qc ranges in january 2012 and disk. Twentyfourth informational supplement abstract the supplemental information presented in this document is. Antifungal susceptibility testing, ul standards pdf, hei standards, engine performance pdf, performance metrics pdf, performanceflatplatevsvacuumtube. Antimicrobial susceptibility of invasive haemophilus influenzae, 2012 the antimicrobial susceptibility of all 60 viable invasive isolates of h. The clinical laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast guidelines are the most popular breakpoint guidelines used in antimicrobial susceptibility testing worldwide. Twentyfirst informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute.
Performance standards for antimicrobial disk and dilution. The clsi publication, performance standards for antimicrobial disk susceptibility tests. Ampicillin, coamoxiclav, cefuroxime and cefaclor minimum inhibitory concentrations mics were determined by etest on haemophilus test medium. Performance standards for antimicrobial disk susceptibility tests, th edition view sample pages this standard covers the current recommended methods for disk susceptibility testing and. Quality assurance for antimicrobial susceptibility testing of neisseria gonorrhoeae in canada, 2003 to 2012 pam sawatzky, a gary liu, a joanne r. Laboratory standards institute antimicrobial susceptibility testing. Background a variety of techniques are available for antimicrobial susceptibility testing of neisseria gonorrhoeae. Setting male and female std clinic of regional std. Clinical and laboratory standards institute document m02a11performance standards for antimicrobial disk susceptibility tests. Atu and information on how to handle unavoidable technical uncertainty in. Clinical breakpoints fungi candida and aspergillus guidance for organisms not listed in breakpoint tables. Testing for these drugs is done using the kirby bauer method at this time, since they are not yet available for testing using the broth microdilution method.
United states department of health and human services. Performance of standards for antimicrobial susceptibility testing ast clsi 2016 presented by. Educational susceptibility testing as a critical component of. Appropriately performed qc procedures monitor a test systems precision i. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory. American proficiency institute 2012 1st test event antimicrobial susceptibility testing ungraded participants were asked to perform antimicrobial susceptibility testing on this k. Mycobacterium tuberculosis drug susceptibility testing program. Performance standards for antimicrobial susceptibility testing, twentyninth edition, m100ed29e. Subcommittee on antimicrobial susceptibility testing since 2012.
June 2010, january 2011, january 2012, january 20, january 2014, january 2015. Mycobacterium tuberculosis drug susceptibility testing. Clsi m07ed11 and m100ed29 package methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, eleventh edition, m07ed11e. The colony counts of the inoculum were performed on drugfree agar medium and. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute clsiapproved standards. Performance standards for antimicrobial susceptibility testing. There are two internationally recognized standard methods for the performance of antifungal susceptibility testing of candida spp. Antimicrobial sensitivity tests, control and prevention. Performance standards for antimicrobial susceptibility testing, 24th informational supplement 24th edition. European committee on antimicrobial susceptibility testing 2012. The average mic agreement between the participating laboratories ranged from 85. Performance standards for antimicrobial disk susceptibility tests, th edition view sample pages this standard covers the current recommended methods for disk susceptibility testing and criteria for quality control testing. Quality assurance for antimicrobial susceptibility testing of. Purpose the purpose of the kirbybauer disk diffusion susceptibility test is to.
This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11a8. The staphylococcus aureus were tested for methicillin susceptibility using 5. In june 2010, the clinical and laboratory standards institute clsi published new mic and disk diffusion interpretive criteria i. In this document, we attempt to answer many of the frequently asked questions regarding antimicrobial susceptibility testing. Dillon, b vanessa allen, c brigitte lefebvre, d linda hoang, e greg tyrrell, f paul van caeseele, g paul levett, h and irene martin a. Laboratory standards institute antimicrobial susceptibility testing standards m02 a12. Quality assurance for antimicrobial susceptibility testing of neisseria gonorrhoeae in canada. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. M02a11, performance standards for antimicrobial disk susceptibility tests. The performance of antimicrobial susceptibility testing programmes.
Comparative assessment of cds, clsi disc diffusion and. The subcommittee has worked diligently to improve m31a2performance standards for antimicrobial disk and dilution. Hakemivala associate prof of sbmu in the name of god panel of. This document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. Application of computers in antimicrobial susceptibility testing 39 10. Use of trade names and commercial sources is for identification only and does not imply.
Approved standard 9th edition, represents the standard for clinical laboratories performing susceptibility testing today. Munro, cls, mt ascp ast qc is a critical component of a quality control plan qcp to ensure reliable patient results. Antimicrobial susceptibility testing for many years, and she is a member of two clsi working. Clinical and laboratory standards institute, wayne, pa. Performance standards for antimicrobial susceptibility. Eucast breakpoint criteria are being adopted by automatic antimicrobial susceptibility testing systems. Standard methods for the detection of antimicrobial resistance. Apr 11, 2016 the clinical laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast guidelines are the most popular breakpoint guidelines used in antimicrobial susceptibility testing worldwide. M100s performance standards for antimicrobial susceptibility testing this document provides updated tables for the clinical and laboratory standards institute. Approved standardeleventh edition includes a series of. The importance of quality control qc for antimicrobial. Progress in antifungal susceptibility testing of candida spp.
Kirbybauer disk diffusion susceptibility test protocol. These changes apply to penicillins, cephalosporins and monobactams, and are. M100 performance standards for antimicrobial susceptibility testing, 30th edition. Objective the aim of this study was to find a costeffective, reliable and easily applicable microbiological method to detect antimicrobial susceptibilities of n. However, investigators must remain cognizant that the performance characteristics of. Twelfth informational supplement mic interpretative standards as. Antimicrobial susceptibility testing of bacteria isolated from aquatic animals and their environments. Approved standard third edition describes the standard agar disk diffusion method, as well as standard. Twentieth informational supplement on free shipping on qualified orders. Standards institute antimicrobial susceptibility testing standards. Etest is a quantitative technique for determining the antimicrobial susceptibility of gramnegative and grampositive aerobic bacteria such as.
Performance standards for antimicrobial susceptibility testing 2001 ferraro, mary jane. Comparative assessment of cds, clsi disc diffusion and etest. Susceptibility tests for bacteria isolated from animals. Errors in interpretation and reporting results 28 7. M02ed performance standards for antimicrobial disk. International standards organisation susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. Assessment of the phoenix automated system and eucast. Results are reported as the mic in micrograms or unitsml. Performance standards for antimicrobial susceptibility testing this document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. Clsi methods development and standardization working group. Performance standards for antimicrobial susceptibility testing clsi. Direct antimicrobial susceptibility testing of positive. Progress in antifungal susceptibility testing of candida. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11.
Effective evaluations of antimicrobial susceptibility tests asts require robust study design. Laboratory methodologies for bacterial antimicrobial susceptibility testing 4 oie terrestrial manual 2012 i disk diffusion, ii broth dilution, iii agar dilution. Carbapenem resistances in enterobacteriaceae, can commercial. The clinical and laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast recently changed their recommendations concerning the interpretation and reporting of in vitro drug susceptibility testing dst results. Quality control in antimicrobial susceptibility testing 29 8. These tests can be useful for screening isolates to categorize them as susceptible, intermediate, resistant, or non. Twentyfifth informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. Comparison of clinical laboratory standards institute and. The clinical and laboratory standards institute clsi subcommittee on antimicrobial susceptibility testing has recognized that many published studies reporting the performance of commercial asts casts suffer from major design andor analysis flaws, rendering the results. Standards for testing the in vitro antimicrobial susceptibility of bacteria that grow anaerobically are found in clsi document m11.
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